Transgenic and Chimeric Mouse Facility

Director: Stephen Liebhaber, MD
Co-Director: Nancy Cooke, MD

The Transgenic and Chimeric Mouse Facility provides expertise in a range of transgenic technologies, enabling the facility to regularly introduce new and improved services. Among the services provided by the Facility are:

  • Generation of transgenic mice via microinjections of DNA into fertilized oocyte
  • Generation of chimeric mice via injection of embryonic stem cells into blastocysts
  • Direct genome editing (Zn finger, TALEN, and CRISPR/Cas9) by microinjection of RNA and DNA into fertilized embryos
  • Embryo re-derivation
  • Embryo and sperm cryopreservation
  • In vitro fertilization
  • Centralized and monitored cryopreservation storage
  • Maintenance of generally useful mouse lines including those for recombination-activation gene expression

The Facility uses state-of-the-art laser conditioning of the zona to facilitate IVF and has intracytoplasmic sperm injection capability on-line to complement the in vitro fertilization service. Newly developed services include the major expansion of cryopreservation services with corresponding expansion of the centralized and dedicated cryopreservation facility, integration of mouse ES microinjection service with a centralized gene knock-out core facility, and direct genome editing by Zn-finger, TALEN and CRISPR-Cas9 technologies.

Contact

Stephen Liebhaber, MD
Phone: 215-898-7834
liebhabe@mail.med.upenn.edu

For more information, you can visit the Facility's website: https://www.med.upenn.edu/genetics/tcmf/