Description of Research Expertise:
Production and characterization of pathogenic human monoclonal auto- and alloantibodies
My laboratory is interested in characterizing the human immune response to auto- and alloantigens on a molecular level in order to understand more about human immune repertoire development. Our goal is to use this information to develop specific therapeutic agents that down-regulate immune responses in the settings of autoimmune disease such as immune hemolytic anemias and thrombocytopenias, transfusion reactions, hemolytic disease of the newborn, and acute rejection of solid organ allografts.
To approach these problems experimentally, we have combined Fab/phage-display technology with a novel cell-surface panning method to isolate large arrays of clinically significant human monoclonal antibodies from individual patients. This approach has enabled us to study the genetic and immunological properties of pathogenic auto- and alloantibodies.
Villa CH., Pan DC., Zaitsev S., Cines D.B., Siegel D.L., Muzykantov, V.R.: Delivery of drugs bound to erythrocytes: new avenues for an old intravascular carrier Therapeutic Delivery 6 : 795-826,2015.
Polak, K., Acierno, M., Raj, K., Mizukamo, K., Siegel, D.L., Giger, U: Dog Erythrocyte Antigen 1 (DEA 1): Mode of inheritance and initial characterization Veterinary Clinical Pathology 44 : 369-379,2015.
Pickens, B., Mao, Y., Li, D., Siegel, D.L., Poncz, M., Cines, D.B., Zheng, X.L.: Platelet-delivered ADAMTS13 inhibits arterial thrombosis and prevents thrombotic thrombocytopenic purpura in murine models Blood 125 : 3326-34,2015.
Casina, V.C., Hu, W., Mao, J., Lu, R.-N., Hanby, H.A., Pickens, B., Kan, Z., Lim, W.K., Mayne, L., Ostertag, E., Kacir, S., Siegel, D.L., Englander, S.W., Zheng, X.L.: High-resolution epitope mapping by HX MS reveals the pathogenic mechanism and a possible therapy for autoimmune TTP syndrome Proc Natl Acad Sci (USA) 112 : 9620-9625,2015.
Zhao A., Tohidkia M.R., Siegel D.L., Coukos G., Omidi Y.: Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy Critical Reviews in Biotechnology 36 : 276-289,2016.
Hammers, C.M., Chen, J., Lin, C., Kacir, S., Siegel, D.L., Payne, A.S., Stanley, J.R.: Persistence of anti-desmoglein 3 IgG+ B-cell clones in pemphigus patients over years J Invest Dermatol 135 : 742-749,2015.
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