Production and characterization of pathogenic human monoclonal auto- and alloantibodies
My laboratory is interested in characterizing the human immune response to auto- and alloantigens on a molecular level in order to understand more about human immune repertoire development. Our goal is to use this information to develop specific therapeutic agents that down-regulate immune responses in the settings of autoimmune disease such as immune hemolytic anemias and thrombocytopenias, transfusion reactions, hemolytic disease of the newborn, and acute rejection of solid organ allografts.
To approach these problems experimentally, we have combined Fab/phage-display technology with a novel cell-surface panning method to isolate large arrays of clinically significant human monoclonal antibodies from individual patients. This approach has enabled us to study the genetic and immunological properties of pathogenic auto- and alloantibodies.
Villa C., Pan D., Zaitsev S., Siegel D.L., Muzykantov, V.: Coupling drugs to carrier erythrocytes: A resurrection of an (almost) forgotten approach for vascular drug delivery Therapeutic Delivery : 2015.
Pickens, B., Mao, Y., Li, D., Siegel, D.L., Poncz, M., Cines, D.B., Zheng, X.L.: Platelet-delivered ADAMTS13 protects against arterial thrombosis and antibody-mediated acquired thrombotic thrombocytopenic purpura Blood 10.1038/nm.3910 : 2015.
Siegel D.L., Westhoff, C: Rh and LW Blood Groups Rossi’s Principles of Transfusion Medicine : 2015.
Hammers, C., Chen, J., Lin, C., Kacir, S., Siegel, D.L., Payne, A.S., Stanley, J.R.: Persistence of anti-desmoglein 3 IgG+ B-cell clones in pemphigus patients over years J Invest Dermatol 135 : 742-749,2015.
Gao, C., Liu, Y., Zhang, H., Zhang, Y., Fukuda, M.N., Palma, A.S., Kozak, R.P., Childs, R.A., Nonaka, M., Li, Z., Siegel, D.L., Hanfland, P., Peehl, D.M., Chai, W., Greene, M.I., Feizi, T.: Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array J. Biol. Chem. 289 : 16462-16477,2014.
O'Doherty, U., Siegel, D.L.: Transfusion Reactions The Intensive Care Manual, 2nd edition : 450-456,2014.
Zhao A., Tohidkia M.R., Siegel D.L., Coukos G., Omidi Y.: Phage antibody display libraries: a powerful antibody discovery platform for immunotherapy Critical Reviews in Biotechnology 10.3109/07388551.2014.958978 : 1-14,2014.
Azzato E., Gentile C., Carpenter H., Siegel D., Watt C.: Successful red blood cell gemotyping from leukoreduced packed red blood cell segments abstracts of the 2014 Annual Meeting of the Association for Molecular Pathology : 2014.
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